Cell neurobiology techniques

de Alan A. Boulton, Glen B. Baker

Cell Neurobiology Techniques is the second work updating and expanding the best-selling inaugural volume of Humana Press's warmly received Neuromethods series, General Neurochemical Techniques (vol. 1). The cutting-edge techniques detailed in this new edition include those that are particularly popular in multidisciplinary neuroscience research. There are readily reproducible methods for establishing neural cell cultures, measuring enzymes and their inhibitors, and using quantitative autoradiography to study monoamine uptake sites and receptors in the brain. Additional methods cover the use of flow cytometry to study developmental neurobiology, applications of magnetic resonance spectroscopy (MRS) to human brain metabolism, and the study of drug metabolism. The companion volumes, In Vivo Neuromethods and In Vitro Neurochemical Techniques, review both in vivo methods and in vitro neurochemical and molecular neurobiological approaches. Like the original, all three cutting-edge works will prove exceptionally useful to those basic and clinical neuroscientists who want to expand the range of their current research or develop competence in complementary methods.