A Laboratory Manual
Edición de la obra Manipulating the mouse embryo
| Autor | Marina Gertsenstein, Kristina Vintersten, Richard Behringer |
|---|---|
| Editorial | Cold Spring Harbor Laboratory Press |
| Fecha de publicación | December 15, 2002 |
| Idioma | inglés |
| Páginas | 764 |
| Formato | Hardcover |
| ISBN-13 | 9780879695743 |
| ISBN-10 | 0879695749 |
| OCLC | 50725188 |
| LCCN | 2002034976 |
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| Número de Cutter | G384m |
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Provides background information and detailed protocols for developing a mouse colony and using the animals in transgenic and gene-targeting experiments. The protocols list the animals, equipment, and reagents required and step-by-step procedures. Topics include in vitro culture of preimplantation embryos, surgical procedures, the production of chimeras, and the analysis of genome alterations. The third edition adds protocols for cloning mice, modifying embryonic stem cells, intracytoplasmic sperm injection, and cryopreservation of embryos.